Journal: Journal of Virology

Article Title: A CRISPR Activation Screen Identifies Genes That Protect against Zika Virus Infection

doi: 10.1128/JVI.00211-19

Figure Lengend Snippet: Immunofluorescence of IFI6- and IFN-λ2-expressing ZIKV-infected cells. (A) Huh7 cell lines expressing IFI6 or nontargeting sgRNAs were stained with an IFI6-specific antibody Scale bar = 20 μm. (B) Medium from Huh7 cell lines expressing IFN-λ2 or NT sgRNAs was used to treat Huh7 cells. After 18 h, the cells were harvested and analyzed by qPCR using Mx1 primers. Recombinant IL-28b (IFN-λ3, R-IL-28B) was used as a positive control (10 ng/μl). ***, P < 0.01; ****, P < 0.001, one-way ANOVA Dunnett’s multiple-comparison test. (C and D) Huh7 cell lines expressing IFI6, IFN-λ2, or nontargeting sgRNAs were infected with ZIKV (MOI, 10) (C) or DENV (MOI, 1) (D) for 2 h. After 24 h (ZIKV) or 48 h (DENV), the cells were fixed and stained with flavivirus protein E-specific antibody and an antibody recognizing dsRNA. DAPI was used for nucleus staining. Scale bar = 100 μm. The relative mean fluorescence intensity of each image compared to the control was quantified using Fiji and is presented below each image.

Article Snippet: Interferon alpha B2 was purchased from PBL (NJ; catalog no. 11115-1), and recombinant human interleukin 28B (IL-28B)/IFN-λ 3 protein was from R&D Systems (catalog no. 5259-IL). . sgRNA library amplification and lentivirus production.

Techniques: Immunofluorescence, Expressing, Infection, Staining, Recombinant, Positive Control, Comparison, Fluorescence, Control

Journal: Cell

Article Title: Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19

doi: 10.1016/j.cell.2020.04.026

Figure Lengend Snippet: Transcriptional and Serological Profile of Clinical COVID-19 Patients (A) Volcano plot depicting DEGs in post-mortem lung samples of two COVID-19 patients compared with healthy lung biopsies. DEGs (p-adjusted < 0.05) with a |log 2 (fold change)| of more than 2 are indicated in red. Non-significant DEGs with a |log 2 (fold change)| of more than 2 are indicated in green. (B and C) Cytokine profiles of COVID-19 patients. Sera of 24 COVID-19 patients and 24 SARS-CoV-2-negative controls were analyzed by ELISA for the protein levels of (B) IFN-I and IFN-III or (C) a broad panel of cytokines. The dotted line depicts the limit of detection. Statistical significance was calculated by Mann-Whitney non-parametric t test. NS, non-significant; ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0001.

Article Snippet: VeriKine-HS human IFN-λ 1/2/3 serum ELISA kit , PBL Interferon Source , Cat# 61840-1.

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Cell

Article Title: Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19

doi: 10.1016/j.cell.2020.04.026

Figure Lengend Snippet:

Article Snippet: VeriKine-HS human IFN-λ 1/2/3 serum ELISA kit , PBL Interferon Source , Cat# 61840-1.

Techniques: Virus, Plasmid Preparation, Recombinant, Enzyme-linked Immunosorbent Assay, Software

Journal: Cell Reports

Article Title: HSV-1 and Zika Virus but Not SARS-CoV-2 Replicate in the Human Cornea and Are Restricted by Corneal Type III Interferon

doi: 10.1016/j.celrep.2020.108339

Figure Lengend Snippet:

Article Snippet: Recombinant Mouse IL-28B/IFN Lambda 3 , PBL assay science , Cat# 12820-1.

Techniques: Control, Virus, Recombinant, RNAscope, Software